Introduction The test kit is detecting for the quantitative analysis of Total Aflatoxin in cereal, feed, peanuts. The Total Aflatoxin mainly infect corn, peanuts, nuts and etc. principle of test The Total Aflatoxin (AFT) ELISA Test Kit is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take caloration with TMB substrate. The samples absorbance value is an inverse relationship with AFT content. Lastly, compared with the standard curve can be concluded the AFT residues in sample. Technique Data Kit sensitivity: 0.02ppb (ng/ml) reactive mode: 25 , 30min 15min Detection Limits: Sample Detection Limits Cereal 0.1 ppb Compound feed 0.2ppb Sauces, Wheat, Other feed 0.2ppb Edible oil, peanut 0.2ppb Beer 0.2 ppb Wine, soy, vinegar 0.1 ppb Cross-reaction rate: Aflatoxin B1 . .100% Aflatoxin B2 .. .75% Aflatoxin G1 .. .100% Aflatoxin G2 .97% Aflatoxin M1 .. .30% Sample Recovery rate: Cereal, Compound feed .. . 85 15% Peanut .. 82 15% Edible oil .. 85 15% Sauces, Wheat, Other feed .. 83 15% Beer .. 84 15% Wine, soy, vinegar .. 87 15%
The Total Aflatoxin mainly infect corn, peanuts, nuts and etc. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate will bind with the primary antibody that is complexed to the toxin coated on the plate wells, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with AFT content. Lastly, compared with the standard curve can be concluded the AFT residues in sample.
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Nanjing Pars Biochem Co., Ltd is a premier leather products export company, established in 2010 and based in China. With a decade of experience, Nanjing Pars Biochem Co., Ltd has built a reputation for delivering high-quality leather goods to clients across the globe. The company specializes in a wide range of leather products, including bags, wallets, belts, and custom leather accessories, all crafted with precision and attention to detail. Leveraging advanced manufacturing techniques and stringent quality control processes, Nanjing Pars Biochem Co., Ltd ensures that each product meets the highest standards. The company’s commitment to excellence, timely delivery, and customer satisfaction has made it a trusted name in the international market.Nanjing Pars Biochem Co., Ltd continues to expand its global footprint, exporting its superior leather products to various countries, while maintaining strong relationships with clients worldwide.
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