COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR
- Estimate pricing$ 2
- AvailabilityIn stock
- SKUKN002
- Limit of detection500 copies/ml
- Result time1.5 hour
- Kit contents48 Tests /Kit
Products Description
Description This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour. Kit Contents (48 Tests /Kit) Dual Enzyme solution A 50 µl Dual Ncvo-O / N reaction solution A 1 ml Dual Ncvo-O / N positive control A 200 µl Dual Negative control A 200µl Applicable equipment ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments. Specimens requirements Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc. Basic Protocol 1. Sample preparation. Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C. 2. Reaction mixture preparation According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control. The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally. Components Volume Dual Enzyme solution A 1 µl Dual Ncvo-O / N reaction solution A 19µl Final Volume: 20 µl 3. Sample adding. Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly. 4. Perform quantitative PCR Place the reaction tube inside a real-time PCR instrument. Set the channel and sample information, reaction system volume 25 µl. Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N. Perform quantitative PCR using recommended cycling parameters settings: Step Temperature Time Number of cycles Fluorescence Detector 1 50C 15 min 1 Off 2 95C 3 min 1 Off 3 95C 10sec 45 Off 60C 30 sec On Result analysis: 1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly. 2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).
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Products Specification
- SKUKN002
- Limit of detection500 copies/ml
- Result time1.5 hour
- Kit contents48 Tests /Kit
- Applicable equipmentABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments
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Buy Online Wholesale COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR from Supplier & Exporter in China
This COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR is a diagnostic kit designed for the identification of ORF1ab and N genes of 2019-nCoV using fluorescence quantitative probe-based PCR. It offers a limit of detection as low as 500 copies/ml and provides results within 1.5 hours. The kit contains dual enzyme solution, reaction solution, positive and negative controls, and is applicable for use with ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments.
Import Wholesale COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR for Your Business Needs
At Global Trade Plaza, we understand that quality is paramount in the B2B sector. As updated by the seller, the COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR is manufactured to meet rigorous industry standards, ensuring reliability and performance for your business operations. Designed with businesses in mind, the product offers outstanding end user experience. As your business grows, so do your needs, hence it is designed to scale with you, offering flexible solutions that adapt to changing demands. Explore more such product varieties from sellers, suppliers, manufacturers and exporters at our website. You can even import or export COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR or connect with global Importers and Exporters online.
About Supplier
Aivd Biotech is a premier leather products export company, established in 2010 and based in China. With a decade of experience, Aivd Biotech has built a reputation for delivering high-quality leather goods to clients across the globe. The company specializes in a wide range of leather products, including bags, wallets, belts, and custom leather accessories, all crafted with precision and attention to detail. Leveraging advanced manufacturing techniques and stringent quality control processes, Aivd Biotech ensures that each product meets the highest standards. The company’s commitment to excellence, timely delivery, and customer satisfaction has made it a trusted name in the international market.Aivd Biotech continues to expand its global footprint, exporting its superior leather products to various countries, while maintaining strong relationships with clients worldwide.
Supplier Details for COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR
- Established - 6 Oct, 2026
- Business Type - Contact Seller
- Deals in Product Type - Diagnostic Kits
- Country - China
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Aivd Biotech, a reliable B2B trader from China, dispatches COVID-19 Dual Nucleic Acid Detection Kit RT-qPCR primarily through the port at China. Packaging types (e.g., bulk, custom, eco-friendly) and estimated pricing may vary based on order volume and destination. To get detailed logistics and pricing information, reach out via the Inquire Now button or Sign Up for direct supplier interaction.
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